Quantitative Optical Coherence Tomography for Longitudinal Monitoring of Postnatal Retinal Development in Developing Mouse Eyes

A better study of postnatal retinal development is essential for the in-depth understanding nature vision system. To date, quantitative analysis primarily limited to endpoint histological examination. This validate in vivo optical coherence tomography (OCT) longitudinal monitoring developing mouse eyes. OCT images C57BL/6J mice were recorded from day (P) 14 P56. Three-dimensional (3D) frame registration and super averaging adopted investigate fine structure retina. Quantitative revealed distinct outer inner layer changes, corresponding eye development. At retina, external limiting membrane (ELM) ellipsoid zone (EZ) band intensities gradually increased with aging, IZ was detectable by P28. a hyporeflective (HRL) between nerve fiber (NFL) plexiform (IPL) observed eyes disappeared aging. Further image individual RGCs within HRL young However, merged NFL IPL aged Moreover, sub-IPL be enhanced interpret kinetics, model based on eyeball expansion, cell apoptosis, structural modification proposed.